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Patch clamp recordings from membranes which contain gap junction channels.

P R Brink¹, S F Fan

¹Department of Anatomical Sciences, School of Medicine, State University of New York, Stony Brook 11794.

Biophysical Journal

|September 1, 1989

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Researchers identified two ion channels in earthworm giant axons. A 100 pS channel exhibits properties consistent with gap junction channels, crucial for neuronal communication.

Area of Science:

Neuroscience
Cell Biology
Ion Channel Physiology

Background:

Gap junctions facilitate direct cell-to-cell communication in the nervous system.
Understanding the specific ion channels involved in earthworm giant axons is key to comprehending their rapid signal transmission.

Purpose of the Study:

To characterize ion channels present in the septal membranes of earthworm median and lateral giant axons.
To identify channels with properties consistent with gap junction function.

Main Methods:

Patch-clamp recordings were performed on the exposed cytoplasmic side of earthworm giant axon septal membranes.
Recordings were made in both attached and detached patch configurations.
Ion selectivity and conductance were assessed using various ions and blockers.

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Main Results:

Two distinct channel types were observed: a K+ channel (30-40 pS, TEA/Cs+ sensitive) and a larger conductance channel (90-110 pS).
The larger channel (approx. 100 pS) conducted multiple ions (Cs+, K+, Na+, TMA+, Cl-, TEA+) and was largely unaffected by common blockers.
This 100 pS channel demonstrated voltage insensitivity, but its opening probability was reduced by H+ and Ca2+.

Conclusions:

The characterized 100 pS channel exhibits properties highly indicative of a gap junction channel in earthworm giant axons.
These findings contribute to understanding the molecular basis of electrical coupling in invertebrate giant axon systems.