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Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
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Simultaneous imaging of neural activity in three dimensions
Sean Quirin1, Jesse Jackson1, Darcy S Peterka1
1Department of Biological Sciences, Columbia University New York, NY, USA.
Frontiers in Neural Circuits
|April 29, 2014
Abstract:
We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro and in vivo. This method, combining structured illumination with volume projection imaging, could be used as a technological platform for brain activity mapping.

