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Related Experiment Videos

Carbohydrate composition of beta-core.

D L Blithe1, R E Wehmann, B C Nisula

  • 1Developmental Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.

Endocrinology
|November 1, 1989
PubMed
Summary
This summary is machine-generated.

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The carbohydrate structure of beta-core, a pregnancy urine molecule, was analyzed. Beta-core lacks terminal sugars found in hCG beta, suggesting a truncated N-linked oligosaccharide structure.

Area of Science:

  • Biochemistry
  • Glycobiology
  • Hormone Analysis

Background:

  • Beta-core is a key component of human chorionic gonadotropin (hCG) related molecules in pregnancy urine.
  • Previous studies deduced partial carbohydrate structure of beta-core using lectin binding.
  • Recent advancements enable detailed carbohydrate composition analysis.

Purpose of the Study:

  • To determine the detailed carbohydrate composition of beta-core and hCG beta (CR119).
  • To elucidate structural differences in glycosylation between beta-core and hCG beta.
  • To understand the implications for beta-core's biological function and detection.

Main Methods:

  • Direct compositional analysis of sialic acid and neutral sugars after trifluoroacetic acid hydrolysis.
  • High-performance liquid chromatography (HPLC) on a Dionex CarboPac column with pulsed amperometric detection (PAD).

Related Experiment Videos

  • Quantification of monosaccharides relative to peptide content.

    • Main Results:

    • Both beta-core and hCG beta possess two N-linked oligosaccharides per molecule.
    • hCG beta has additional O-linked oligosaccharides, absent in beta-core.
    • Beta-core shows significantly lower sialic acid, galactose, and N-acetylgalactosamine compared to hCG beta.
    • The trimannosyl-chitobiose core of N-linked glycans is intact in beta-core.
    • Similar fucose content suggests comparable fucosylation of internal N-acetylglucosamine in both molecules.

    Conclusions:

    • Beta-core's N-linked oligosaccharides resemble hCG beta's but lack terminal sialic acid, galactose, and N-acetylglucosamine.
    • These structural differences explain the distinct properties and potential functions of beta-core.
    • The findings provide insights into hCG heterogeneity during pregnancy.