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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
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Genomic Analysis of Invasive Human Bone Marrow Derived Mesenchymal Stem Cells.

Lesley A Mathews1, Elaine M Hurt2, Xiaohu Zhang3

  • 1Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD 20892, USA.

Journal of Bone Marrow Research
|April 29, 2014
PubMed
Summary

Human mesenchymal stem cells (hMSCs) gene expression during invasion was studied. Key genes involved in development and immunity were identified, offering insights for therapeutic applications.

Keywords:
Gene expressionInvasionSTAT3hMSCs

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Area of Science:

  • Cell Biology
  • Stem Cell Research
  • Molecular Biology

Background:

  • Human bone marrow-derived mesenchymal stem cells (hMSCs) possess multipotent differentiation capabilities.
  • hMSCs have broad therapeutic potential, but their gene expression profiles are not fully understood.
  • Recent research focuses on elucidating gene expression in hMSCs and their differentiated forms.

Purpose of the Study:

  • To investigate gene expression patterns during hMSC invasion through basement membranes.
  • To identify specific genes and pathways involved in the invasive behavior of hMSCs.

Main Methods:

  • Gene expression analysis was performed on invasive and non-invasive hMSCs.
  • Agilent gene expression arrays and Matrigel invasion assays were utilized.
  • Cells were cultured in a specialized stem cell medium (SCM).

Main Results:

  • 435 genes were upregulated twofold or more in invasive hMSCs.
  • Upregulated genes included developmental regulators (NANOG, STAT3, STAT5A) and immune/inflammatory pathways (EZH2, SUZ12, HIF-1α).
  • STAT3 inhibition using Static reversed the invasion process.

Conclusions:

  • The study enhances understanding of genetic pathways governing hMSC invasion.
  • Findings contribute to the development of hMSC-based therapeutic strategies.