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Related Experiment Video

Updated: Apr 30, 2026

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

A simple, rapid one-step ELISA using antibody-antibody complex.

Wenwen Jiang¹, Xiaoli Liu, Di Wu

¹The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, People's Republic of China.

Biotechnology and Applied Biochemistry

|April 30, 2014

Summary

A novel one-step enzyme-linked immunosorbent assay (ELISA) significantly speeds up detection and improves sensitivity. This optimized method allows for faster, more sensitive detection of trace antigens (Ag) in biological samples and on surfaces.

Keywords:

HSA antibody biomaterials one-step ELISA protein signal

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Last Updated: Apr 30, 2026

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

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Published on: April 6, 2021

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An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

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Published on: March 14, 2016

Area of Science:

Biochemistry
Analytical Chemistry
Immunology

Background:

Enzyme-linked immunosorbent assay (ELISA) is a widely used technique in diagnostics and research.
Traditional ELISA methods are often time-consuming, limiting their efficiency.
There is a need for faster and more sensitive immunoassay techniques.

Purpose of the Study:

To develop a one-step ELISA method for faster and more sensitive detection of trace antigens.
To optimize the antibody-antigen complex formation for improved assay performance.
To evaluate the performance of the one-step ELISA on modified surfaces.

Main Methods:

Preparation of a primary antibody-secondary antibody complex (Ab-Ab complex).
Optimization of the mole ratio of primary to secondary antibodies.
Application of the one-step ELISA for detecting proteins on poly(N-vinylpyrrolidone) (PVP)-modified silicon surfaces (Si-PVP).

Main Results:

Achieved a detection sensitivity of 1 ng/mL, an improvement over traditional ELISA.
Reduced assay operating time by 30%.
Demonstrated controllable signal intensity by adjusting antibody ratios or color development time.
Obtained results comparable to radiolabeling methods when detecting proteins on Si-PVP surfaces.

Conclusions:

The developed one-step ELISA offers a rapid and sensitive method for detecting trace amounts of antigen (Ag).
This technique is suitable for detecting proteins in solution and on low-adsorption material surfaces.
The one-step ELISA shows significant promise for clinical diagnostic applications involving trace antigen detection.