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A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood

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Equine autologous platelet concentrates: A comparative study between different available systems.

L N Hessel¹, G Bosch, P R van Weeren

¹Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.

Equine Veterinary Journal

|April 30, 2014

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Autologous platelet concentrates (APCs) vary significantly in equine healing applications. Differences in platelet and growth factor concentrations across methods impact tissue regeneration, requiring careful selection for horses.

Area of Science:

Veterinary regenerative medicine
Equine sports medicine
Biotechnology and biomaterials

Background:

Autologous platelet concentrates (APCs) are increasingly used in horses for tissue regeneration.
Existing APC systems, often adapted from human medicine, lack specific validation and characterization for equine use.
This gap necessitates an evaluation of current APC preparation methods in horses.

Purpose of the Study:

To compare haematological parameters and growth factor concentrations in equine APCs.
To evaluate 5 different preparation techniques: 1 filtration-based and 4 centrifugation-based methods.
To assess the in vitro performance of these APC systems in horses.

Main Methods:

An in vitro experiment using blood samples from 6 horses.

Keywords:

comparison growth factors horse platelet concentrates platelet-rich plasma tissue regeneration

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Production of APCs using 5 distinct commercial and non-commercial techniques.

Measurement of platelet, leucocyte, platelet-derived growth factor-BB (PDGF-BB), and transforming growth factor-β1 (TGF-β1) concentrations and enrichment factors.

Main Results:

All APC systems increased platelet concentration, but significant enrichment was observed in only two.
Leukocyte counts varied widely, with one method reducing them to 9% and others increasing them significantly.
Growth factor enrichment differed substantially between systems, ranging from 184% to 1255% for PDGF-BB and 93% to 560% for TGF-β1.

Conclusions:

Significant variations in haematological and biochemical characteristics exist among equine APC preparation techniques.
These differences may influence clinical outcomes and tissue regeneration quality in horses.
Clinicians should exercise caution and not solely rely on human-derived data when selecting APC methods for equine applications.