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An enzyme immunoassay for prostate-specific p30 antigen detection in the postcoital vaginal tract
Journal - Forensic Science Society
|July 1, 1989
Abstract:
A sandwich enzyme immunoassay test utilising a mouse monoclonal antibody has been adapted for the sensitive detection of the p30 prostatic antigen in semen and in postcoital vaginal swabs. In liquid semen, p30 was still detectable at a 1/1,000,000 dilution, and it could still be detected on vaginal swabs 24 h postcoitus.
Insights
A sensitive new test can detect prostate-specific antigen (PSA) in semen and on vaginal swabs up to 24 hours after intercourse. This advance aids in forensic analysis and understanding semen presence in sexual assault cases.
Area of Science:
- Forensic Science
- Biochemistry
- Immunology
Background:
- Prostate-specific antigen (PSA) is a protein produced by the prostate gland.
- Sensitive detection of PSA is crucial for forensic investigations involving semen identification.
Purpose of the Study:
- To adapt a sensitive sandwich enzyme immunoassay for the detection of PSA.
- To evaluate the detectability of PSA in semen and postcoital vaginal swabs.
Main Methods:
- Utilized a sandwich enzyme immunoassay.
- Employed a mouse monoclonal antibody for PSA detection.
- Tested samples of liquid semen and vaginal swabs collected postcoitus.
Main Results:
- The assay demonstrated high sensitivity, detecting PSA in liquid semen at a 1/1,000,000 dilution.
- PSA was detectable on vaginal swabs up to 24 hours after sexual intercourse.
Conclusions:
- The developed immunoassay is highly sensitive for PSA detection.
- This method is effective for identifying semen in forensic samples, including postcoital vaginal swabs, for extended periods.