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Related Experiment Videos

HLA-DR molecules on all cells are electrophoretically unique.

J M Pesando1, P Hoffman, M A Stucki

  • 1Fred Hutchinson Cancer Research Center, Seattle, WA.

Leukemia Research
|January 1, 1989
PubMed
Summary

Human leukemia cells (ALL) display unique HLA-DR molecules compared to normal B cells. This difference is inherent to the leukemic cells, not an artifact of cell culture or EBV transformation.

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Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Oncology

Background:

  • Previous studies suggested differences in Human Leukocyte Antigen - Antigen D Related (HLA-DR) molecules between acute lymphoblastic leukemia (ALL) and normal B cells.
  • These observed differences were potentially attributed to artifacts from cell lines, Epstein-Barr Virus (EBV) transformation, or cell culture conditions.

Purpose of the Study:

  • To validate the observed electrophoretic differences in HLA-DR molecules between in vivo leukemic cells and autologous normal B cells.
  • To determine if cell line artifacts, EBV transformation, or cell culture influence HLA-DR molecule expression.

Main Methods:

  • Surface iodination of leukemic and autologous normal peripheral blood B cells from four ALL patients.
  • Comparison of HLA-DR molecule electrophoretic patterns using SDS-PAGE.
  • Analysis of HLA-DR molecule electrophoretic patterns on normal tonsil B cells before and after EBV transformation.

Main Results:

  • Electrophoretic patterns of HLA-DR molecules on in vivo leukemic cells were identical to those on corresponding cell lines.
  • In vivo leukemic cells exhibited an extra beta chain band compared to autologous normal B cells.
  • EBV transformation did not alter the electrophoretic appearance of HLA-DR molecules on normal B cells.

Conclusions:

  • The observed electrophoretic differences in HLA-DR molecules between ALL and normal B cells are genuine and present in vivo.
  • Cell lines are reliable models for studying the chemical basis of these HLA-DR molecular differences in leukemia.
  • This finding supports the use of cell lines for further investigation into the unique immunogenetic characteristics of ALL.

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