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The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
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Related Experiment Video

Updated: Apr 30, 2026

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
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Human AdV-specific T cells: persisting in vitro functionality despite lethal irradiation.

R Geyeregger1, C Freimüller1, J Stemberger1

  • 1Department of Clinical Cell Biology and FACS Core Unit, Children's Cancer Research Institute (CCRI), Vienna, Austria.

Bone Marrow Transplantation
|April 30, 2014
PubMed
Summary
This summary is machine-generated.

Irradiated donor lymphocytes (DLIs) may retain antiviral activity in immunocompromised patients. This study shows that even after irradiation, human adenovirus (HAdV)-specific T cells remain viable and functional, supporting their potential use in transplantation.

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Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
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Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
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Area of Science:

  • Immunology
  • Transplantation Medicine
  • Virology

Background:

  • Viral infections like human adenovirus (HAdV) and cytomegalovirus (CMV) pose life-threatening risks for immunocompromised patients post-allogeneic hematopoietic stem cell transplantation (HSCT).
  • Adoptive immunotherapy using virus-specific T cells offers clinical benefits but faces challenges due to high costs and regulatory hurdles.
  • Unmanipulated donor lymphocyte infusions (DLIs) risk graft-versus-host disease (GvHD), limiting their use for antiviral therapy.

Purpose of the Study:

  • To investigate the effect of irradiation on the viability and functionality of in vitro-expanded human adenovirus (HAdV)-specific T cells.
  • To assess the potential of irradiated HAdV-specific T cells for use in adoptive immunotherapy following HSCT.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) were stimulated with HAdV-peptide pools and IL-15 for 12 days to expand HAdV-specific T cells.
  • Expanded cells were irradiated with 30 Gy, mimicking the dose used for granulocyte concentrates.
  • Cell viability and polyfunctional activity were assessed 48 hours post-irradiation using flow cytometry.

Main Results:

  • Approximately 15.6% of the expanded HAdV-specific T cells remained viable and cytolytically active 48 hours after irradiation.
  • The data suggest that irradiation does not completely abolish the functionality of these virus-specific T cells.

Conclusions:

  • Irradiated HAdV-specific T cells retain a degree of viability and cytotoxic function, supporting previous hypotheses about their potential role in managing viral infections post-HSCT.
  • These findings may pave the way for exploring the use of irradiated DLIs as a safer alternative for antiviral immunotherapy in HSCT recipients.