Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.9K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.9K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.9K
Cell Migration01:09

Cell Migration

16.6K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
16.6K
Cell Migration01:19

Cell Migration

6.1K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.1K
Master Transcription Regulators02:23

Master Transcription Regulators

6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

2.5K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ectodermal loss of ARHGAP29 alters epithelial morphology and disrupts murine palatogenesis.

Development (Cambridge, England)·2026
Same author

Correction: Epidemiology and Clinicopathologic Features with Prognostic Implications of Conventional Ameloblastoma: A 22-Year Retrospective Study.

Head and neck pathology·2026
Same author

Mast cell homeostasis depends on KIT ligand from dermal fibroblasts and perivascular cells in mouse skin.

The Journal of investigative dermatology·2026
Same author

Epidemiology and Clinicopathologic Features with Prognostic Implications of Conventional Ameloblastoma: A 22-Year Retrospective Study.

Head and neck pathology·2026
Same author

Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells.

Nature cell biology·2025
Same author

Piezo1 balances membrane and cortex tension to stabilize intercellular junctions and maintain the epithelial barrier.

Journal of cell science·2025

Related Experiment Video

Updated: Apr 30, 2026

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

7.3K

Interferon regulatory factor 6 regulates keratinocyte migration.

Leah C Biggs1, Rachelle L Naridze2, Kris A DeMali3

  • 1Department of Pediatrics, The University of Iowa, Iowa City, IA 52242, USA Interdisciplinary Graduate Program in Genetics, The University of Iowa, Iowa City, IA 52242, USA.

Journal of Cell Science
|April 30, 2014
PubMed
Summary

Interferon regulatory factor 6 (Irf6) is crucial for skin cell migration. Irf6 deficiency impairs wound healing by affecting cell movement and adhesion through the RhoA pathway.

Keywords:
Interferon regulatory factor 6KeratinocytesMigration

More Related Videos

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
08:57

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

9.9K
A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

11.7K

Related Experiment Videos

Last Updated: Apr 30, 2026

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

7.3K
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
08:57

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

9.9K
A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

11.7K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Dermatology

Background:

  • Interferon regulatory factor 6 (Irf6) is known to regulate keratinocyte proliferation and differentiation.
  • Its role in cellular migration and adhesion remains less understood.

Purpose of the Study:

  • To investigate the function of Irf6 in regulating cellular migration and adhesion.
  • To elucidate the molecular mechanisms underlying Irf6's role in these processes.

Main Methods:

  • Analysis of Irf6-deficient embryos and murine embryonic keratinocytes.
  • In vitro scratch-wound healing assays with live imaging.
  • Investigation of cell-matrix adhesions and cytoskeletal organization (stress fibers, RhoA activity).
  • Pharmacological inhibition of ROCK (a RhoA effector) and assessment of Arhgap29 expression.

Main Results:

  • Irf6-deficient embryos exhibited delayed wound closure.
  • Irf6-deficient keratinocytes showed impaired directional migration and reduced speed in scratch-wound assays.
  • These cells were larger, more spread, and displayed increased stress fibers and RhoA activity.
  • ROCK inhibition rescued the impaired wound healing, and Arhgap29 expression was reduced in Irf6-deficient cells.

Conclusions:

  • Irf6 plays a significant role in regulating keratinocyte migration and wound healing.
  • The RhoA signaling pathway is a key mediator of Irf6's function in cellular migration.
  • Irf6 influences cell adhesion and cytoskeletal dynamics, impacting cell movement.