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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

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Published on: December 31, 2017

Terminal RFLP analysis to determine the oral microbiota with hyposalivation.

Y Hayashi¹, T Saito, T Ohshima

¹R&D Department, Sunstar Inc., 5-30-1, Kamihamuro, Takatsuki, Osaka, 569-1044, Japan, yoshiko.hayashi@jp.sunstar.com.

Archives of Microbiology

|April 30, 2014

Summary

This summary is machine-generated.

Reduced saliva (hyposalivation) significantly alters tongue coating microbiota composition, favoring specific bacterial genera. This study highlights saliva

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Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

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Published on: October 20, 2011

Area of Science:

Microbiology
Oral Health
Genomics

Background:

Hyposalivation (reduced salivary secretions) may increase oral pathogens.
The impact of hyposalivation on the overall oral microbiota, including uncultivable bacteria, is not well understood.

Purpose of the Study:

To investigate the relationship between hyposalivation and oral microbiota composition.
To analyze the oral microbiota in hyposalivation using terminal restriction fragment length polymorphism (T-RFLP) of 16S rDNA.

Main Methods:

Analyzed tongue coating microbiota from 61 subjects using T-RFLP.
Grouped subjects into hyposalivation and normo-salivation groups.
Assessed salivary volume, Candida albicans counts, and dental status.

Main Results:

Microbiota profiles clustered into two distinct groups based on T-RFLP patterns.
Cluster I, associated with hyposalivation, showed higher proportions of Veillonella, Dialister, Prevotella, Fusobacterium, and Streptococcus.
Salivary volume significantly influenced microbial community composition.

Conclusions:

Hyposalivation demonstrably alters the oral microbial community structure.
T-RFLP analysis effectively revealed shifts in both cultivable and uncultivable oral bacteria due to reduced saliva.
Salivary volume plays a critical role in maintaining oral microbiota balance.