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Published on: January 12, 2020
Overexpression of NOTCH-regulated ankyrin repeat protein is associated with breast cancer cell proliferation
Tatsuhiko Imaoka1, Tomomi Okutani, Kazuhiro Daino
14-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. t_imaoka@nirs.go.jp.
Background/Aim:
NOTCH-regulated ankyrin repeat protein (NRARP) has been implicated in crosstalk between NOTCH and wingless-type mouse mammary tumor virus integration site (WNT) signals during development. Our study aimed to clarify its role in breast cancer cells.
Materials And Methods:
Public microarray data were used to analyze gene expression in human and rat breast cancer. A short interfering RNA was introduced into MCF7 and T47D human breast cancer cells for NRARP silencing. Gene expression was analyzed by quantitative polymerase chain reaction.
Results:
The NRARP transcript was commonly overexpressed in various rat mammary cancer models. In addition, a subset of human breast cancer also expressed high levels of NRARP transcript, which correlated positively with up-regulation of cell proliferation-related genes. Silencing of NRARP suppressed the growth of MCF7 and T47D cells and lowered the expression of cell cycle-related genes in MCF7 cells.
Conclusion:
NRARP may stimulate cell proliferation in human breast cancer.
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