Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

11.1K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
11.1K
Labeling DNA Probes03:31

Labeling DNA Probes

7.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triplet-Mediated Photon Upconversion via Near-Infrared-II Excitation without Tetracene Derivatives.

Journal of the American Chemical Society·2026
Same author

In Situ Activating a Protein-Complexed Quenched Probe via Alkaline Phosphatase Response for Pyroptosis-Mediated Cancer Immunotherapy.

Angewandte Chemie (International ed. in English)·2026
Same author

Programmable In Vivo Synthesis of Quantum Dots.

Angewandte Chemie (International ed. in English)·2026
Same author

Beyond-1000 nm low-energy sunlight-driven photocatalysis enabled by quantum dot-based photon upconversion.

National science review·2026
Same author

Simultaneous Localization of Electrons/Holes by Surface Cationic Dangling-Bonds/Vacancies for Synergistically Boosting Photocatalytic Activity.

Journal of the American Chemical Society·2026
Same author

Efficient near-infrared-excitable quantum dot-based triplet-triplet annihilation upconversion with a record anti-Stokes shift <i>via</i> low coverage of mono-styryl-BODIPY ligands.

Chemical science·2026

Related Experiment Video

Updated: Apr 30, 2026

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
09:04

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

7.0K

Uniform fluorescent nanobioprobes for pathogen detection.

Ling-Hong Xiong1, Ran Cui, Zhi-Ling Zhang

  • 1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, State Key Laboratory of Virology, and Wuhan Institute of Biotechnology, Wuhan University , Wuhan 430072, People's Republic of China.

ACS Nano
|May 1, 2014
PubMed
Summary

Researchers engineered Staphylococcus aureus into fluorescent cellular beacons for sensitive pathogen detection. This novel nanobioprobe approach simplifies cell surface modification for diverse biomedical applications.

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K
Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues
08:01

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

1.5K

Related Experiment Videos

Last Updated: Apr 30, 2026

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
09:04

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

7.0K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K
Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues
08:01

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

1.5K

Area of Science:

  • Biotechnology
  • Nanomaterials Science
  • Microbiology

Background:

  • Synthesizing novel nanomaterials often requires complex methods not found in nature.
  • Cellular factories offer a promising avenue for producing unique nanomaterials.
  • Previous work utilized yeast cells for nanoparticle synthesis.

Purpose of the Study:

  • To transform Staphylococcus aureus into uniform, highly fluorescent, and photostable cellular beacons.
  • To develop a facile method for creating fluorescent nanobioprobes using these engineered cells.
  • To demonstrate the utility of these nanobioprobes for detecting pathogens.

Main Methods:

  • Engineered Staphylococcus aureus to express fluorescent proteins, creating cellular beacons.
  • Utilized the surface protein A of S. aureus for antibody conjugation, avoiding genetic modification.
  • Integrated immunomagnetic beads for enhanced targeting and detection capabilities.

Main Results:

  • Successfully created uniform, fluorescent, and photostable Staphylococcus aureus cellular beacons.
  • Developed a straightforward method to fabricate fluorescent nanobioprobes via protein A-antibody interaction.
  • Demonstrated specific detection of H9N2 avian influenza virus (AIV) with a limit of 8.94 ng/mL.
  • Showcased the adaptability of the system for detecting various pathogens by changing antibodies.

Conclusions:

  • Staphylococcus aureus can be effectively engineered into versatile cellular beacons for nanobioprobe development.
  • This method provides a simplified and efficient platform for creating targeted nanobioprobes.
  • The developed nanobioprobes show significant potential for sensitive pathogen detection and broader biomedical applications.