Ochratoxin A in several grains in Iran
Hamed Reza Beheshti1, Mohammad Asadi
1a Testa Quality Control Laboratory , North-East Food Industrial Technology and Biotechnology Park , Mashhad , Iran.
Summary
Ochratoxin A was found in 32% of green gram, 13.3% of chickpea, 10% of lentil, and 17.5% of wheat flour. However, all detected Ochratoxin A contamination levels remained below European Union and Iranian regulatory limits.
Area of Science:
- Food Science
- Analytical Chemistry
- Mycotoxicology
Background:
- Ochratoxin A (OTA) is a significant mycotoxin found in various food commodities.
- Grain and derived products are susceptible to OTA contamination, posing potential health risks.
- Accurate quantification of OTA is crucial for food safety and regulatory compliance.
Purpose of the Study:
- To determine the Ochratoxin A content in a range of grain and derived products.
- To assess the level of OTA contamination in commonly consumed food items.
- To compare detected OTA levels against established regulatory limits.
Main Methods:
- Analysis of 100 grain and derived product samples.
- High-performance liquid chromatography (HPLC) coupled with immunoaffinity column (IAC) clean-up.
- Fluorometric detection for sensitive and specific OTA quantification.
Main Results:
- Ochratoxin A was detected in 32% of green gram, 13.3% of chickpea, 10% of lentil, and 17.5% of wheat flour samples.
- The method achieved a high recovery rate of 97%.
- The limit of detection (LOD) was determined to be 0.12 ng g⁻¹.
Conclusions:
- While OTA is present in some grain products, contamination levels were below the regulatory limits set by the European Union and Iran.
- The analytical method employed is effective for accurate OTA determination in food matrices.
- Findings support the safety of the analyzed grain products concerning Ochratoxin A based on current regulations.
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