Related Experiment Video
Updated: Apr 30, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
Nitrite in hamburgers in Arak, Iran
Mohammad Rezaei1, Nabi Shariatifar, Gholamreza Jahed Khaniki
1a Department of Food Safety and Hygiene, School of Public Health , Tehran University of Medical Sciences , Tehran , Iran.
Nitrite was detected in 85.7% of hamburgers in Arak city, Iran. Residual nitrite levels ranged from 30-100 mg/kg, indicating potential issues with production standards for this meat additive.
Area of Science:
- Food Science
- Analytical Chemistry
- Public Health
Background:
- Nitrite and nitrate are common meat additives for color, flavor, and microbial protection.
- Excessive nitrite/nitrate intake poses health risks, including potential carcinogenicity.
- Organic and natural food standards prohibit chemical preservatives like nitrite/nitrate.
Purpose of the Study:
- To quantify residual nitrite levels in hamburgers sold in Arak city, Iran.
- To assess compliance with food safety standards regarding nitrite use in hamburgers.
Main Methods:
- Random sampling of 105 hamburger specimens.
- Analysis of residual nitrite using the Official AOAC Method 973.
Main Results:
- Nitrite was detected in 85.7% of the analyzed hamburger samples.
- Residual nitrite concentrations ranged from 30 to 100 mg/kg (p < 0.001).
Conclusions:
- The widespread presence of nitrite in hamburgers suggests non-compliance with regulations, particularly for products not intended to be cured.
- Findings indicate potentially poor production conditions and inadequate sodium nitrite standards in local hamburger manufacturing.
- Further investigation and stricter oversight are recommended to ensure food safety and consumer health.
More Related Videos
Related Concept Videos
2° Amines to N-Nitrosamines: Reaction with NaNO2
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
Physical Properties of Amines
Electrophilic Aromatic Substitution: Nitration of Benzene
Acid Mine Drainage
Inorganic Nitrogen Assimilation

