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Updated: Apr 30, 2026

Characterization of Aquatic Biofilms with Flow Cytometry
Published on: June 6, 2018
Detachment and flow cytometric quantification of seagrass-associated bacteria
Stacey Trevathan-Tackett1, Peter Macreadie1, Peter Ralph1
1Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia.
Abstract:
A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×10(5) and 8.0×10(6)cells mg(-1) DW tissue.
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