Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

3.0K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
3.0K
Colonisation of Pathogens01:25

Colonisation of Pathogens

77
Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...
77
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

10.2K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
10.2K
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

105.4K
Overview
105.4K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

1.1K
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
1.1K
Carrier-Mediated Transport01:06

Carrier-Mediated Transport

1.6K
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA synthesis: When amino acids act as a template.

Current biology : CB·2026
Same author

Mycobacterium tuberculosis VadK is required for the regulation of the methylcitrate cycle and virulence.

EMBO reports·2026
Same author

Redox regulated auto-processing controls delivery of an antibacterial cysteine peptidase toxin.

bioRxiv : the preprint server for biology·2026
Same author

How custom polymerases are driving innovation in synthetic biology.

Current opinion in chemical biology·2026
Same author

A periplasmic protein complex mediates arabinofuranosyltransferase activity and intrinsic drug resistance in <i>Mycobacterium tuberculosis</i>.

Science advances·2026
Same author

Bacteria deliver a microtubule-binding protein into mammalian cells to promote colonization.

Science (New York, N.Y.)·2026

Related Experiment Video

Updated: Apr 30, 2026

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
06:37

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

Published on: February 7, 2013

16.1K

Heme uptake in bacterial pathogens.

Heidi Contreras1, Nicholas Chim1, Alfredo Credali1

  • 1Department of Molecular Biology and Biochemistry, UCI, Irvine, CA 92697, USA.

Current Opinion in Chemical Biology
|May 1, 2014
PubMed
Summary

Bacterial pathogens use diverse strategies to steal heme, an essential nutrient, from human hosts. This review details novel mechanisms for heme uptake and iron release by bacteria.

More Related Videos

Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

11.4K
Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
10:34

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

Published on: February 1, 2018

10.3K

Related Experiment Videos

Last Updated: Apr 30, 2026

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
06:37

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

Published on: February 7, 2013

16.1K
Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

11.4K
Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
10:34

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

Published on: February 1, 2018

10.3K

Area of Science:

  • Microbiology
  • Biochemistry
  • Structural Biology

Background:

  • Iron is vital for all organisms.
  • Bacterial pathogens require iron, often acquiring it via heme from hosts.
  • Heme uptake pathways are crucial for bacterial survival and virulence.

Purpose of the Study:

  • To review recent structural and biochemical data on bacterial heme uptake.
  • To provide mechanistic insights into heme sequestration, transport, and degradation.
  • To highlight the diversity of bacterial heme acquisition strategies.

Main Methods:

  • Structural analysis of heme-binding and transport proteins.
  • Biochemical assays to study heme sequestration and release.
  • Comparative analysis of diverse bacterial heme uptake pathways.

Main Results:

  • Bacterial pathogens employ specialized secreted or membrane proteins to capture host heme.
  • Heme transport across bacterial membranes involves divergent pathways with novel protein structures.
  • Intracellular heme degradation liberates iron and distinct byproducts via sequence-divergent enzymes.

Conclusions:

  • Bacterial heme uptake pathways are highly diverse, showcasing varied protein components and mechanisms.
  • Understanding these pathways offers insights into bacterial pathogenesis and potential therapeutic targets.
  • The breakdown of heme within bacteria yields iron and unique degradation products.