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Counting Proteins in Single Cells with Addressable Droplet Microarrays
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Robust and versatile ionic liquid microarrays achieved by microcontact printing.

Christian A Gunawan1, Mengchen Ge1, Chuan Zhao1

  • 1School of chemistry, The University of New South Wales, Sydney, New South Wales 2052, Australia.

Nature Communications
|May 1, 2014
PubMed
Summary
This summary is machine-generated.

Ionic liquids and microcontact printing create versatile open microreactors for lab-on-a-chip devices. This approach overcomes solvent volatility and mass transport issues for diverse micro- and nano-scale applications.

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Area of Science:

  • Materials Science
  • Chemistry
  • Engineering

Background:

  • Lab-on-a-chip devices offer advantages due to micro- to nano-scale material properties.
  • Challenges in microfluidic chemistry include solvent volatility and reproducibility.
  • Ionic liquids present a non-volatile alternative for microfluidic applications.

Purpose of the Study:

  • To develop a facile protocol for high-throughput fabrication of open microreactors and microfluidics.
  • To combine ionic liquids with microcontact printing for advanced microfluidic systems.
  • To address limitations of solvent volatility and mass transport in lab-on-a-chip devices.

Main Methods:

  • Utilized microcontact printing to pattern non-volatile ionic liquid droplets.
  • Developed open microreactors and microfluidic systems using ionic liquids.
  • Investigated applications in electrochemistry, microfabrication, and sensor arrays.

Main Results:

  • Demonstrated micropatterned ionic liquid droplets as functional electrochemical cells.
  • Showcased applications in microfabrication of metals and charge transfer complexes.
  • Successfully employed ionic liquid systems for protein immobilization and amperometric gas sensor arrays.

Conclusions:

  • Ionic liquid-based microfluidics offer a versatile platform for overcoming traditional lab-on-a-chip limitations.
  • The developed protocol facilitates high-throughput fabrication of microreactors.
  • Miniaturized ionic liquid systems provide solutions for solvent volatility and mass transport in microfluidic devices.