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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
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General principles of cryopreservation.

Roger G Gosden1

  • 1Weill Cornell Medical College, 1300 York Avenue, New York, NY, 10065, USA, roger.gosden@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|May 1, 2014
PubMed
Summary
This summary is machine-generated.

Cryopreservation is vital for assisted reproduction, enabling gamete and embryo banking for infertility treatment and fertility preservation. Automation is key to improving the consistency and reliability of these crucial cryopreservation techniques.

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Area of Science:

  • Reproductive medicine and cryobiology.

Background:

  • Cryopreservation is essential for assisted reproductive technology (ART).
  • It supports infertility treatment and fertility preservation for at-risk patients.
  • Banking of gametes, embryos, and gonadal tissues is a key application.

Purpose of the Study:

  • To highlight the importance of cryopreservation in ART.
  • To discuss the evolution and current state of cryopreservation protocols.
  • To emphasize the potential of automation in improving cryopreservation outcomes.

Main Methods:

  • Review of established cryopreservation techniques, including slow/equilibrium cooling and vitrification.
  • Discussion of the empirical development of current protocols.
  • Exploration of the role of operator skill in cryopreservation success.

Main Results:

  • Cryopreservation significantly enhances the effectiveness of ART cycles.
  • Existing protocols are heavily reliant on the skill of the practitioner.
  • Automation presents a pathway to greater reproducibility.

Conclusions:

  • Cryopreservation is a cornerstone technology in modern reproductive medicine.
  • Further automation of cryopreservation methods is needed to ensure uniform and reliable results.
  • Advancements in automation will enhance patient care in fertility treatment and preservation.