Related Experiment Video
Updated: Apr 30, 2026

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Published on: February 8, 2015
Far-red organic fluorophores contain a fluorescent impurity
Matthew B Stone1, Sarah L Veatch
1Department of Biophysics, University of Michigan, 930 N University, Ann Arbor MI 48109 (USA).
Abstract:
Far-red organic fluorophores commonly used in traditional and super-resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near-red emission. This near-red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super-resolution localization microscopy.
More Related Videos
Related Concept Videos
Immunofluorescence Microscopy
Variables Affecting Phosphorescence and Fluorescence
Fluorescence and Phosphorescence: Instrumentation
Photoluminescence: Applications
Super-resolution Fluorescence Microscopy

