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[Quantitative determination of voglibose contents in its tablets with high-performance liquid chromatography-mass
Xiang-qun Zhang1, Ling-hua Wu1, Fang-jun Chen1
1Chiatai Qingchunbao Pharmaceutical Co. Ltd, Hangzhou 310023, China.
Objective:
To develop a method for determination of voglibose contents in its tablets by high-performance liquid chromatography-mass spectrometry (HPLC-MS).
Methods:
The measurements were carried out on an Agilent ZORBAX Eclipse Plus C18 column (2.1×150mm 3.2μm) with a temperature of 40 degrees Celsius. A mixture of methanol and water (2:3,v/v) was used as a mobile phase at a flow rate of 0.25 ml/min. Voglibose was detected in an electrospray ionization (ESI) mode with MRM.
Results:
The calibration curves of voglibose showed good linearity in a range of 1.5804-2.6340 μg/ml (r=0.9990). The average recovery was 100.2% with RSD of 1.37% (n=6) for m/z 268.2/74.2.Linearity was obtained with r=0.9976 and the average recovery was 99.3% with RSD of 1.78% (n=6) for m/z 268.2/92.2.
Conclusion:
HPLC-MS method is accurate,reproducible and can be used for quality control of voglibose tablets.
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