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[Preliminary application of the immunogold-silver staining technique in diagnosing thromboangiitis obliterans]
In this paper we reported the preliminary application of immunogold-silver staining (IGSS) technique in immunopathological diagnosis of thromboangiitis obliterans (TAO). We used IGSS technique to detect the immune complexes deposit in the vascular walls (18 cases) and the anti-vascular autoantibody in serum (28 cases) of TAO patients. The immune complexes deposited in tissue were found in 100%, and the anti-vascular autoantibody in 86% of TAO patients. The results of IGSS technique were compared with that of immunofluorescent staining, immunoenzyme staining and avidin-biotin-peroxidase complex staining techniques. It was demonstrated that both the sensitivity and specificity of IGSS technique were significantly higher than those of other techniques.
In this paper we reported the preliminary application of immunogold-silver staining (IGSS) technique in immunopathological diagnosis of thromboangiitis obliterans (TAO). We used IGSS technique to detect the immune complexes deposit in the vascular walls (18 cases) and the anti-vascular autoantibody in serum (28 cases) of TAO patients. The immune complexes deposited in tissue were found in 100%, and the anti-vascular autoantibody in 86% of TAO patients. The results of IGSS technique were compared with that of immunofluorescent staining, immunoenzyme staining and avidin-biotin-peroxidase complex staining techniques. It was demonstrated that both the sensitivity and specificity of IGSS technique were significantly higher than those of other techniques.