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Updated: Apr 30, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
The PI3K pathway acting on alternative HIV-1 pre-mRNA splicing
Frank Hillebrand1,2, Steffen Erkelenz1, Nora Diehl1
1Institute for Virology, Universitaetsklinikum Duesseldorf, Universitaetsstr. 1, D-40225 Duesseldorf, Germany.
Abstract:
HIV-1 mediates pro-survival signals and prevents apoptosis via the phosphatidylinositol-3-kinase (PI3K) pathway. This pathway, however, also affects phosphorylation of serine-arginine (SR) proteins, a family of splicing regulatory factors balancing splice site selection. We now show that pharmacologic inhibition of PI3K signalling alters the HIV-1 splicing pattern of both minigene- and provirus-derived mRNAs. This indicates that HIV-1 might also promote PI3K signalling to balance processing of its transcripts by regulating phosphorylation of splicing regulatory proteins.
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