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[Stable solution of fuchsin--a new method for preparation of Ziehl-Neelsen staining]
L F Siqueira1, R G de Almeida, W Francisco
1Departamento de Epidemiologia da Faculdade de Saúde Pública da Universidade de São Paulo.
Hansenologia Internationalis
|June 1, 1987
Abstract:
The fuchsin salts currently available in Latin American market have shown some instability when in solution, according to the classic Ziehl-Neelsen method, resulting in a total precipitation of the salt. The authors indicate a new technique for the preparation of this solution, in order to minimize the action of interfering factors responsible for the precipitation, obtaining thus a greater solubility of the salt, as well as the solution stability. The method effectiveness is reinforced by the utilization of a smaller amount of the salt and the attainment of a larger storage period for the solution.