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Related Experiment Videos

Of matrices and men.

P G Righetti1

  • 1Chair of Biochemistry, Faculty of Pharmacy, University of Milan, Italy.

Journal of Biochemical and Biophysical Methods
|July 1, 1989
PubMed
Summary
This summary is machine-generated.

This review surveys modern electrokinetic matrices, focusing on cellulose acetate, agarose, and polyacrylamide gels. It highlights advancements in modified matrices and new amphiphatic materials for electrophoresis and chromatography.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Electrokinetic techniques rely on various matrices for separation and analysis.
  • Cellulose acetate, agarose, and polyacrylamide gels are the primary matrices used.
  • Advancements in matrix technology are crucial for improving separation efficiency and applicability.

Purpose of the Study:

  • To provide a comprehensive survey of matrices used in modern electrokinetic techniques.
  • To review the properties and applications of cellulose acetate, agarose, and polyacrylamide gels.
  • To explore novel and modified matrices for enhanced electrophoretic and chromatographic separations.

Main Methods:

  • Literature review of scientific publications and product information.

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  • Analysis of matrix properties, including pore structure, chemical modifications, and stability.
  • Evaluation of newly developed monomers and composite matrices.

    • Main Results:

    • Cellulose acetate is mainly used in clinical chemistry.
    • Agarose gels form large-pore structures, with several modified brands available.
    • Polyacrylamide gels offer diverse properties due to various crosslinkers and modified monomers.
    • New amphiphatic matrices show promise for advanced separation techniques.

    Conclusions:

    • The field of electrokinetic matrices is rapidly evolving with diverse applications.
    • Modified agaroses and polyacrylamides offer tailored properties for specific separation needs.
    • Emerging amphiphatic matrices represent the latest developments in electrophoresis and chromatography.