Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolated inflammatory process of the caecum.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2025
Same author

Stacking spectral lines with multiplexed Bragg gratings in an acousto-optical tunable filter.

Optics express·2024
Same author

Strangulation ileus caused by malposition of the ureter.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2024
Same author

InSPECtor: an end-to-end design framework for compressive pixelated hyperspectral instruments.

Applied optics·2023
Same author

Vector-apodizing phase plate coronagraph: design, current performance, and future development [Invited].

Applied optics·2021
Same author

Effect of sample type and the use of high or low fishmeal diets on bacterial communities in the gastrointestinal tract of Penaeus monodon.

Applied microbiology and biotechnology·2021

Related Experiment Video

Updated: Apr 30, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.8K

Snapshot coherence-gated direct wavefront sensing for multi-photon microscopy.

T I M van Werkhoven, J Antonello, H H Truong

    Optics Express
    |May 3, 2014
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new wavefront sensing method for multi-photon microscopy. It corrects optical aberrations in turbid media like biological tissues, enabling deeper imaging.

    More Related Videos

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    9.8K
    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
    10:20

    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

    Published on: September 5, 2019

    7.9K

    Related Experiment Videos

    Last Updated: Apr 30, 2026

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.8K
    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    9.8K
    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
    10:20

    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

    Published on: September 5, 2019

    7.9K

    Area of Science:

    • Biomedical Optics
    • Microscopy
    • Adaptive Optics

    Background:

    • Deep imaging in scattering media like biological tissue is limited by scattering and optical aberrations.
    • Adaptive optics (AO) offers a promising solution for correcting these aberrations.
    • Existing wavefront sensing methods may face challenges in turbid environments.

    Purpose of the Study:

    • To develop and validate a novel wavefront sensing scheme for multi-photon scanning microscopes.
    • To enable deeper penetration and clearer imaging in scattering biological tissues.
    • To correct both system-induced and sample-induced optical aberrations.

    Main Methods:

    • Utilized back-reflected light from the sample within a multi-photon scanning microscope.
    • Employed coherence gating and a confocal pinhole to isolate light from a specific depth.
    • Interfered back-reflected light with a tilted reference beam to generate a fringe pattern.
    • Analyzed fringe pattern distortions in the Fourier domain to extract wavefront aberrations.
    • Integrated a virtual Shack-Hartmann sensor for aberration smoothing and modal representation.

    Main Results:

    • Successfully demonstrated wavefront sensing using reflected light in turbid media.
    • Corrected system-induced aberrations from a deformable mirror (DM).
    • Corrected sample-induced aberrations in biological samples, including rat tail collagen fibers.
    • Showcased aberration correction in a Hoechst-stained MCF-7 cancer cell spheroid.

    Conclusions:

    • The presented wavefront sensing scheme is effective for aberration correction in multi-photon microscopy.
    • This technique facilitates deeper and clearer imaging in scattering biological tissues.
    • The method shows potential for various applications in deep tissue imaging and optical microscopy.