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Published on: August 30, 2017
Fluorescent polyion complex nanoparticle that incorporates an internal standard for quantitative analysis of protein
Takanobu Nobori1, Shujiro Shiosaki, Takeshi Mori
1Department of Applied Chemistry, Faculty of Engineering, ‡Graduate School of Systems Life Sciences, §Center for Future Chemistry, ||International Research Center for Molecular Systems, ⊥Center for Advanced Medical Innovation, and #Innovation Center for Medical Redox Navigation, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Abstract:
We demonstrate a polyion complex (PIC) nanoparticle that contains both a responsive fluorophore and an "internal standard" fluorophore for quantitative measurement of protein kinase (PK) activity. The PK-responsive fluorophore becomes more fluorescent with PK-catalyzed phosphorylation of substrate peptides incorporated in the PIC, while fluorescence from the internal standard remains unchanged during phosphorylation. This new concept will be useful for quantitative PK assays and the discovery of PK inhibitors.
