Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

1.8K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
1.8K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

1.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
1.8K
Whole Body Regeneration01:33

Whole Body Regeneration

3.6K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
3.6K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

9.1K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.1K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

8.3K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiscale in vivo imaging of tumor evolution using a germline conditional triple-reporter mouse.

Nature biotechnology·2026
Same author

Apparent diffusion coefficient and kurtosis parameters show early response to anti-angiogenic therapy in patients with colorectal liver metastases.

Cancer imaging : the official publication of the International Cancer Imaging Society·2026
Same author

A comprehensive pharmacological survey across heterogeneous patient-derived glioblastoma stem cell models.

iScience·2026
Same author

Targeting RUNX1 protects against diastolic dysfunction in a two-hit mouse model of heart failure with preserved ejection fraction.

Cardiovascular research·2026
Same author

Runx1 and Runx2 act in concert to suppress Wnt/β-catenin-driven mammary tumourigenesis.

British journal of cancer·2026
Same author

Discovery of a Highly Potent and Selective mTOR Inhibitor that Strongly Suppresses Glioblastoma Multiforme Cell Growth.

Journal of medicinal chemistry·2026

Related Experiment Video

Updated: Apr 30, 2026

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
06:40

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Published on: April 1, 2021

4.3K

c-Src drives intestinal regeneration and transformation.

Julia B Cordero1, Rachel A Ridgway1, Nicola Valeri2

  • 1The Beatson Institute for Cancer Research, Bearsden Glasgow, UK.

The EMBO Journal
|May 3, 2014
PubMed
Summary

The non-receptor tyrosine kinase c-Src (Src) drives intestinal stem cell proliferation in colorectal cancer. This kinase is essential for tissue self-renewal, regeneration, and tumor initiation, with a non-redundant role in the mouse intestine.

Keywords:
ApcSrcintestinal stem cellsregenerationtumourigenesis

More Related Videos

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
07:48

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Published on: November 21, 2015

19.0K
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.4K

Related Experiment Videos

Last Updated: Apr 30, 2026

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
06:40

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Published on: April 1, 2021

4.3K
Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
07:48

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Published on: November 21, 2015

19.0K
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.4K

Area of Science:

  • Molecular Biology
  • Oncology
  • Developmental Biology

Background:

  • Non-receptor tyrosine kinase c-Src (Src) is frequently overexpressed or activated in human cancers.
  • The specific role of Src in colorectal cancer (CRC) has been unclear due to redundant family members.
  • Src amplification and activating mutations occur in up to 20% of human CRC tumors.

Purpose of the Study:

  • To define the role of Src in intestinal tissue homeostasis, regeneration, and hyperplasia.
  • To investigate Src's function in intestinal stem cell (ISC) proliferation and tumor initiation.
  • To determine if Src's role in the intestine is redundant or non-redundant.

Main Methods:

  • Utilized adult Drosophila and mouse intestinal epithelium as model systems.
  • Employed genetic gain and loss of function experiments.
  • Analyzed signaling pathways including EGFR, Ras/MAPK, and Stat3.

Main Results:

  • Src is both necessary and sufficient to drive ISC proliferation during self-renewal, regeneration, and tumor formation.
  • Src plays a non-redundant role in the mouse intestine, not substitutable by Fyn or Yes kinases.
  • Src upregulates EGFR and activates Ras/MAPK and Stat3 signaling pathways to promote ISC proliferation.

Conclusions:

  • Src plays a critical, non-redundant role in intestinal stem/progenitor cell proliferation.
  • Src is essential for initiating tumor formation in the intestine.
  • Src represents a potential therapeutic target in colorectal cancer.