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Published on: September 22, 2017
72 hours standby time of wet-primed cardiopulmonary bypass circuits: a microbiological quality assurance study
Sebastian Schulz-Stübner1, Cornelia Schorer2, Jürgen Ennker2
1Deutsches Beratungszentrum für Hygiene (BZH GmbH), Freiburg, Germany.
Abstract:
In a microbiological sample study of 15 wet-primed cardiopulmonary bypass circuits in standby mode for 72 hours under regular clinical conditions, no contamination of the priming fluid or the connectors could be detected. Hand contact surfaces of the machines demonstrated environmental microorganisms. These findings indicate the safe use of primed cardiopulmonary bypass circuits in standby mode for 72 hours. A surface disinfection of hand contact surfaces immediately before use is recommended.
Insights
Primed cardiopulmonary bypass circuits are safe for 72-hour standby use, showing no fluid or connector contamination. Surface disinfection of hand contact areas before use is advised to prevent environmental microorganism transfer.
Area of Science:
- Microbiology
- Medical Devices
- Cardiopulmonary Bypass
Background:
- Cardiopulmonary bypass (CPB) circuits are critical during cardiac surgery.
- Maintaining sterility of CPB circuits in standby is essential for patient safety.
- Understanding potential contamination risks in primed circuits is crucial.
Purpose of the Study:
- To assess the microbiological safety of wet-primed cardiopulmonary bypass circuits.
- To determine contamination levels in priming fluid and connectors after 72 hours in standby.
- To evaluate the presence of microorganisms on machine surfaces.
Main Methods:
- Microbiological sampling of priming fluid and connectors from 15 CPB circuits.
- Circuits were in standby mode for 72 hours under clinical conditions.
- Analysis of hand contact surfaces for environmental microorganisms.
Main Results:
- No microbial contamination was detected in the priming fluid or connectors.
- Environmental microorganisms were identified on hand contact surfaces of the machines.
- The integrity of the sterile field within the circuit was maintained.
Conclusions:
- Wet-primed cardiopulmonary bypass circuits can be safely used after 72 hours in standby.
- Surface disinfection of frequently touched areas is recommended prior to use.
- This practice minimizes the risk of introducing environmental contaminants.

