72 hours standby time of wet-primed cardiopulmonary bypass circuits: a microbiological quality assurance study

Sebastian Schulz-Stübner1, Cornelia Schorer2, Jürgen Ennker2

  • 1Deutsches Beratungszentrum für Hygiene (BZH GmbH), Freiburg, Germany.

Insights

Primed cardiopulmonary bypass circuits are safe for 72-hour standby use, showing no fluid or connector contamination. Surface disinfection of hand contact areas before use is advised to prevent environmental microorganism transfer.

Area of Science:

  • Microbiology
  • Medical Devices
  • Cardiopulmonary Bypass

Background:

  • Cardiopulmonary bypass (CPB) circuits are critical during cardiac surgery.
  • Maintaining sterility of CPB circuits in standby is essential for patient safety.
  • Understanding potential contamination risks in primed circuits is crucial.

Purpose of the Study:

  • To assess the microbiological safety of wet-primed cardiopulmonary bypass circuits.
  • To determine contamination levels in priming fluid and connectors after 72 hours in standby.
  • To evaluate the presence of microorganisms on machine surfaces.

Main Methods:

  • Microbiological sampling of priming fluid and connectors from 15 CPB circuits.
  • Circuits were in standby mode for 72 hours under clinical conditions.
  • Analysis of hand contact surfaces for environmental microorganisms.

Main Results:

  • No microbial contamination was detected in the priming fluid or connectors.
  • Environmental microorganisms were identified on hand contact surfaces of the machines.
  • The integrity of the sterile field within the circuit was maintained.

Conclusions:

  • Wet-primed cardiopulmonary bypass circuits can be safely used after 72 hours in standby.
  • Surface disinfection of frequently touched areas is recommended prior to use.
  • This practice minimizes the risk of introducing environmental contaminants.