Influence of DMF-induced oxidative stress on membrane and periplasmic proteins in Paracoccus sp. SKG
B Kirankumar1, Guruprasad B Kulkarni, S Sanjeevkumar
1Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India.
Abstract:
The present study describes the N,N-dimethylformamide (DMF)-induced oxidative stress in Paracoccus sp. SKG. The oxidative stress was evaluated by analysing membrane and periplasmic proteins and K+ efflux, as well as by monitoring the activities of antioxidant enzymes like catalase, superoxide dismutase (SOD) and glutathione S-transferase (GST). The exposure of bacterial cells to a higher concentration of DMF resulted in the modification of membrane fatty acid composition which is accompanied by K+ efflux. Further, this oxidative stress resulted in increased periplasmic protein which can be attributed to the induction of GST and methionine sulphoxide reductase (Msr) enzymes under solvent stress. Paracoccus sp. SKG is tolerant to high concentrations of DMF up to 6% (v/v) and its toxic effects. DMF concentration-dependent induction of GST and Msr activities advocates the significant role of these enzymes in the bacterial defence system. The present study provides information which helps us to understand the ROS scavenging machinery in bacteria. The high tolerance of Paracoccus sp. SKG to DMF can be efficiently explored for various bioremediation and biotransformation applications.
Insights
Paracoccus sp. SKG tolerates N,N-dimethylformamide (DMF) by activating antioxidant enzymes like glutathione S-transferase (GST) and methionine sulphoxide reductase (Msr). This bacterial defense mechanism offers potential for bioremediation applications.
Area of Science:
- Microbiology
- Biochemistry
- Environmental Science
Background:
- N,N-dimethylformamide (DMF) is a common solvent with potential toxicity.
- Understanding bacterial responses to solvent-induced stress is crucial for industrial and environmental applications.
Purpose of the Study:
- To investigate the oxidative stress response of Paracoccus sp. SKG to N,N-dimethylformamide (DMF).
- To identify the key enzymes and mechanisms involved in DMF tolerance in Paracoccus sp. SKG.
- To explore the potential of Paracoccus sp. SKG for bioremediation and biotransformation.
Main Methods:
- Exposure of Paracoccus sp. SKG to varying concentrations of DMF.
- Analysis of membrane and periplasmic proteins.
- Measurement of potassium ion (K+) efflux.
- Assay of antioxidant enzyme activities including catalase, superoxide dismutase (SOD), and glutathione S-transferase (GST).
Main Results:
- DMF exposure induced oxidative stress, altering membrane fatty acid composition and causing K+ efflux.
- Increased periplasmic proteins were observed, linked to the induction of GST and methionine sulphoxide reductase (Msr).
- Paracoccus sp. SKG demonstrated tolerance to DMF up to 6% (v/v), with concentration-dependent induction of GST and Msr.
Conclusions:
- GST and Msr play a significant role in the bacterial defense system against DMF-induced oxidative stress.
- Paracoccus sp. SKG possesses a robust ROS scavenging machinery.
- The high tolerance of Paracoccus sp. SKG to DMF makes it a promising candidate for bioremediation and biotransformation processes.
Related Concept Videos
01:29Biosynthesis of Lipids
01:30Other Stress Responses in Bacteria