Related Experiment Video
Updated: Apr 30, 2026
09:53
In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
Published on: June 15, 2018
7.2K
Cardiomyocyte-specific miRNA-30c over-expression causes dilated cardiomyopathy
Wino J Wijnen1, Ingeborg van der Made2, Stephanie van den Oever2
1Heart Failure Research Center, Academic Medical Center, Amsterdam, The Netherlands; Interuniversitair Cardiologisch Instituut Nederland (ICIN-NHI), Utrecht, The Netherlands.
Plos One
|May 3, 2014
Summary
Overexpressing miRNA-30c in mouse hearts caused dilated cardiomyopathy by impairing mitochondrial function. This study establishes a key in vivo role for microRNA-30c in cardiac physiology and mitochondrial health.
Area of Science:
- Cardiovascular Biology
- Molecular Cardiology
- Mitochondrial Medicine
Background:
- MicroRNAs (miRNAs) are crucial regulators of cellular processes, with their dysregulation linked to heart disease.
- MiRNA-30c's role in cardiac physiology is largely unknown, despite its differential expression during heart failure progression.
Purpose of the Study:
- To investigate the in vivo function of miRNA-30c in the heart.
- To elucidate the mechanisms underlying miRNA-30c's impact on cardiac health.
Main Methods:
- Generation of transgenic mice overexpressing miRNA-30c specifically in cardiomyocytes.
- Phenotypic analysis, gene expression profiling, and protein level assessment of mitochondrial oxidative phosphorylation (OXPHOS) complexes.
Main Results:
- Transgenic mice developed severe dilated cardiomyopathy after 6 weeks of age.
- Early analysis revealed disturbed mitochondrial function and downregulation of OXPHOS complexes III and IV.
- Impaired mitochondrial function due to OXPHOS protein depletion was identified as a potential cause of the cardiomyopathy.
Conclusions:
- MiRNA-30c plays a critical in vivo role in maintaining cardiac physiology.
- Dysregulation of miRNA-30c leads to impaired mitochondrial function and dilated cardiomyopathy.
Related Concept Videos
01:30Cardiomyopathy II: Dilated Cardiomyopathy
790
Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
790
01:25Cardiomyopathy I: Introduction and Classification
826
Cardiomyopathy, or CMP, is a group of diseases affecting the myocardial structure, impairing its ability to pump blood effectively. This condition can lead to arrhythmias, heart failure, or sudden cardiac death.Cardiomyopathies are classified into primary and secondary categories:Primary Cardiomyopathy refers to conditions involving only the heart muscle that are often idiopathic (of unknown cause) or genetic. They primarily affect the myocardium without the involvement of other systemic...
826
01:29Cardiomyopathy III: Hypertrophic Cardiomyopathy
805
Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
805
01:29Cardiomyopathy V: Interprofessional Care
753
Managing cardiomyopathy involves addressing underlying or precipitating causes, treating heart failure with medications, and implementing dietary changes and a balanced exercise and rest regimen.Lifestyle ModificationsCardiomyopathy patients should adopt a low-sodium diet to reduce fluid retention and manage heart failure. A personalized exercise and rest plan helps maintain physical fitness without overstraining the heart. Avoiding alcohol and tobacco is essential to prevent further damage to...
753
01:21Myocarditis I: Introduction
645
Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...
645
01:29Cardiomyopathy IV: Restrictive Cardiomyopathy
952
Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
952