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Updated: Apr 30, 2026
Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
Published on: March 18, 2012
Optimization of fluorometric assay for ozone in solution
Hiromi Nagano1, Gotaro Shiota1, Hidetoshi Arakawa1
1Department of Analytical Biochemistry, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Abstract:
Ozone is widely used for sterilization, deodorization, and cleaning. However, the effects of ozone on human are not well understood, because there is currently no reliable assay method for ozone. Therefore the accurate measurement of ozone is important for its safe use. Here, we report optimized conditions for use with fluorescein derivative 1 (probe 1). Under optimum conditions, probe 1 reacted with ozone quantitatively and specifically to generate a fluorescent substance with an excitation maximum at 493 nm and an emission maximum at 523 nm. We conclude that this assay will be useful for quantifying ozone in solution.
