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[Studies on quality evaluation of Dendrobii devoniani].

Xiao-Na Gan, Ying Xu, Hong Xu

    Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
    |May 6, 2014
    PubMed
    Summary

    This study establishes quality standards for Dendrobii devoniani caulis from Longling, Yunnan, using pharmacognostic and chemical analyses. The developed methods ensure reliable quality control for this important medicinal herb.

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    Area of Science:

    • Pharmacognosy
    • Phytochemistry
    • Analytical Chemistry

    Context:

    • Dendrobii devoniani caulis is a valuable traditional Chinese medicine.
    • Ensuring the quality of medicinal herbs is crucial for therapeutic efficacy and patient safety.
    • Local variations in medicinal plants necessitate region-specific quality standards.

    Purpose:

    • To establish a local quality standard for Dendrobii devoniani caulis sourced from Longling, Yunnan.
    • To develop and validate pharmacognostic and analytical methods for quality control.
    • To determine key quality parameters including polysaccharide and mannose content.

    Summary:

    • Pharmacognostic characteristics, microscopic identification, and Thin-Layer Chromatography (TLC) were employed.
    • Polysaccharide content was quantified using the sulfuric acid-phenol method.
    • High-Performance Liquid Chromatography (HPLC) determined mannose content, while extractive values followed Chinese Pharmacopoeia (2010) guidelines.
    • Results indicated distinct microscopic features, clear TLC spots, and established ranges for polysaccharide (35.7%-52.1%), mannose (27.8%-46.1%), and extractive content (4.5%-10.6%).

    Impact:

    • The developed methods are simple, accurate, reliable, and reproducible.
    • The established quality standard provides a robust framework for evaluating Dendrobii devoniani caulis from Longling, Yunnan.
    • This research contributes to the standardization and quality assurance of herbal medicines.