Stable isotope labeling in mammals (SILAM).
Daniel B McClatchy1, John R Yates
1Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2014
Summary
Stable isotope labeling in mammals (SILAM) enables precise protein quantification in rodents. This technique uses heavy nitrogen-labeled tissues as internal standards for mass spectrometry, advancing disease research and drug target identification.
Area of Science:
- Proteomics
- Biomedical research
- Mass spectrometry
Background:
- Animal models are crucial for understanding human diseases and identifying drug targets.
- Global proteome analysis using mass spectrometry is a key tool in biomedical research.
- Quantitative proteomics requires reliable internal standards for accurate measurements.
Purpose of the Study:
- To introduce and describe the Stable Isotope Labeling in Mammals (SILAM) technique for rodent proteome quantification.
- To highlight the utility of SILAM in disease research and drug discovery.
- To establish a method for accurate protein quantification in animal models.
Main Methods:
- Developed the Stable Isotope Labeling in Mammals (SILAM) method for rodents.
- Achieved complete labeling of all rodent proteins with heavy nitrogen ((15)N).
- Utilized (15)N-labeled rodent tissues as internal standards for quantitative mass spectrometry.
Main Results:
- Successfully implemented SILAM for comprehensive protein labeling in rodents.
- Established a robust method for quantitative proteome analysis using mass spectrometry.
- Demonstrated the application of SILAM-derived internal standards for high-resolution, high-mass-accuracy measurements.
Conclusions:
- SILAM is an effective method for quantitative proteome analysis in rodents.
- This technique significantly enhances the study of animal models for human diseases.
- SILAM facilitates the identification of potential clinical drug targets through precise protein quantification.
