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Related Concept Videos

Tandem Mass Spectrometry01:21

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Updated: Apr 30, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Employing TMT quantification in a shotgun-MS platform.

Darragh P O'Brien1, John F Timms

  • 1Cancer Proteomics Laboratory, EGA Institute for Women's Health, University College London, Room 1.1.09, Gower Street, London, WC1E 6BT, UK.

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Summary

Researchers developed a new method to find early pancreatic cancer biomarkers in blood serum. This approach enriches low-abundance proteins, improving biomarker discovery for complex diseases.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Biomarker Discovery

Background:

  • The blood serum proteome is a rich source of disease biomarkers.
  • Serum complexity requires advanced methods for low-abundance protein quantification.
  • Identifying early disease indicators is crucial for effective treatment.

Purpose of the Study:

  • To discover candidate serum biomarkers for pre-diagnosis pancreatic cancer.
  • To establish a robust workflow for comparative serum proteomic analysis.

Main Methods:

  • Utilized immunodepletion and multi-lectin fractionation to enrich serum proteins.
  • Employed peptide tandem mass tag (TMT) labeling for quantification.
  • Applied SCX and high pH reversed-phase fractionation followed by LC-MS/MS analysis.

Main Results:

  • Successfully compared serum proteomes from pancreatic cancer cases and controls.
  • Demonstrated the workflow's capability in identifying potential protein biomarkers.
  • The method is adaptable for various comparative proteomic studies.

Conclusions:

  • The developed workflow enhances the discovery of low-abundance serum biomarkers.
  • This approach offers a promising strategy for early detection of pancreatic cancer.
  • The methodology is broadly applicable to comparative serum proteomic investigations.