Employing TMT quantification in shotgun-MS proteomic analysis: a focus on skeletal muscle

Quantify proteins present in a sample is the ultimate goal of a proteomic experiment. Although it might reveal countless physiological and /or pathological process on a studied model, it is still a daunting task to perform. Of the protein present in a sample, only a small number can be identified and even a lesser number quantified, each with its own weaknesses and strengths. Presented here are the "tandem mass tags" isobaric labels (TMT) and a protocol for its utilization in the quantification of skeletal muscle, which might be adapted also to other animal tissues.