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Updated: Apr 30, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Published on: September 17, 2016
The use of fluorescent proteins to analyze circadian rhythms
Ekaterina Shor1, Miriam Hassidim, Rachel M Green
1Department of Plant and Environmental Sciences, Hebrew University, Edmund Safra Givat Ram Campus, Jerusalem, 91904, Israel.
Abstract:
Compared with luciferase which is widely used as a reporter for circadian rhythms in Arabidopsis thaliana, available fluorescent markers are generally too stable to allow circadian oscillations to be measured. However, we have developed a technique to use the nuclear localization of circadian-controlled transcription factors fused to a fluorescent reporter as a means of measuring circadian rhythms. This technique has the advantage of being suitable for analyzing rhythms at the level of individual cells and in living plants.
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