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Exploring the third dimension: volume electron microscopy comes of age
Christopher J Peddie1, Lucy M Collinson1
1Electron Microscopy Unit, London Research Institute, Cancer Research UK, London WC2A 3LY, UK.
Micron (Oxford, England : 1993)
|May 6, 2014
Summary
Advances in volume electron microscopy enable detailed 3D cellular visualization. New automated techniques and integrated systems are expanding its use in biomedical research for routine ultrastructural analysis.
Area of Science:
- Cellular Biology
- Microscopy
- Biomedical Research
Background:
- Volume electron microscopy (VEM) is experiencing a resurgence due to technological advancements.
- Traditional serial sectioning techniques are becoming more automated.
- VEM applications are expanding beyond neuroscience.
Purpose of the Study:
- To highlight recent breakthroughs in VEM.
- To discuss the impact of automation on VEM techniques.
- To explore the future potential of VEM in biomedical studies.
Main Methods:
- Development of automated ultramicrotomes and advanced electron microscopes (serial blockface, focused ion beam scanning).
- Integration of light and electron microscopy systems for correlative studies.
- Advancements in specimen preparation for high-resolution 3D imaging.
Main Results:
- Unprecedented detail in 3D specimen visualization is now achievable.
- Automation is making VEM more accessible and efficient.
- Integrated light and electron microscopy allows seamless data acquisition.
Conclusions:
- VEM is poised to become a routine tool for detailed ultrastructural analysis in 3D.
- The integration of imaging modalities will enable comprehensive functional and structural correlations.
- The future of VEM in biomedical research is promising and dynamic.
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