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Updated: Apr 30, 2026
Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease
Published on: December 18, 2016
Proton magnetic resonance spectroscopy of skeletal muscle: a comparison of two quantitation techniques
Xin Wang1, Nouha Salibi2, Laura M Fayad3
1Department of Radiation Oncology, Weill Cornell Medical College, New York, NY 10021, United States.
Rationale And Objectives:
The aim of this study was to develop and compare two methods for quantification of metabolite concentrations in human skeletal muscle using phased-array receiver coils at 3T.
Materials And Methods:
Water suppressed and un-suppressed spectra were recorded from the quadriceps muscle (vastus medialis) in 8 healthy adult volunteers, and from a calibration phantom containing 69mM/L N-acetyl aspartate. Using the phantom replacement technique, trimethylamine specifically [TMA] and creatine [Cr] concentrations were estimated, and compared to those values obtained by using the water reference method.
Results:
Quadriceps [TMA] concentrations were 9.5±2.4 and 9.6±4.1mmol/kg wet weight using the phantom replacement and water referencing methods respectively, while [Cr] concentrations were 26.8±12.2 and 24.1±5.3mmol/kg wet weight respectively.
Conclusions:
Reasonable agreement between water referencing and phantom replacement methods was found, although for [Cr] variation was significantly higher for the phantom replacement technique. The relative advantages and disadvantages of each approach are discussed.
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