Reversible protein inactivation by optogenetic trapping in cells
Sangkyu Lee1, Hyerim Park2, Taeyoon Kyung3
11] Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of Korea. [2] Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. [3].
Abstract:
We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP.
