Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Apr 30, 2026

Author Spotlight: Enhancing Visual Outcomes in Cataract Surgery: A Novel Technique to Prevent Posterior Capsular Opacification Through IOL Rotation

Author Spotlight: Enhancing Visual Outcomes in Cataract Surgery: A Novel Technique to Prevent Posterior Capsular Opacification Through IOL Rotation

Published on: July 7, 2023

Experimental lens capsular bag model for posterior capsule opacification.

Jong Hwa Jun¹, Wern-Joo Sohn, Youngkyun Lee

¹Department of Ophthalmology, School of Medicine, Dongsan Medical Center, Keimyung University, Daegu, Republic of Korea.

Cell and Tissue Research

|May 6, 2014

Summary

A new in vitro model uses low-melting-point agarose gel to study posterior capsule opacification (PCO). This method supports the capsular bag, allowing detailed observation of lens epithelial cell behavior in PCO development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Timing and Associated Factors to Formation and Detection of Sequestrum in Patients With Medication-Related Osteonecrosis of the Jaw.

Oral diseases·2026

Same author

In Vivo Model of Short-Term Efficacy and Favorable Safety of Botulinum Toxin Type E Compared with Type A.

Toxins·2026

Same author

Spectrometer-free time-division multiplexed NIR time-of-flight vision system for visually similar material recognition.

Scientific reports·2026

Same author

Proteomic Characterization of AS1411 Reveals ATP6AP1 as a Mediator of Triple-Negative Breast Cancer Progression.

Proteomics·2026

Same author

A Cross-Species Hydroquinone Model Replicates Smoking-Related Corneal Endothelial Oxidative Injury and Enables Therapeutic Screening of FGF10.

Investigative ophthalmology & visual science·2026

Same author

Comparative analysis of spatial expression patterns of ectopic olfactory receptors.

Genes & genomics·2026

Area of Science:

Ophthalmology
Cell Biology
Biomaterials Science

Background:

Posterior capsule opacification (PCO) is a common complication after cataract surgery.
Existing in vitro models for PCO research have limitations in maintaining natural capsular structure and allowing comprehensive analysis.

Purpose of the Study:

To develop and establish a novel in vitro culture model for investigating posterior capsule opacification (PCO).
To utilize low-melting-point (LMP)-agarose gel to support the capsular bag and mimic the in vivo environment for lens epithelial cells (LECs).

Main Methods:

Whole lens explants from rodent and porcine eyes were embedded in 2% LMP-agarose gel.
Sham cataract surgery (capsulotomy and lens fiber removal) was performed on the explants.
Capsular bag/lens epithelial cell (CB-LEC) complexes were cultured and observed using phase-contrast microscopy and histological examinations.

More Related Videos

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery

Published on: December 1, 2023

Author Spotlight: Advancing Understanding of Age-Related Lens Stiffness Changes

Author Spotlight: Advancing Understanding of Age-Related Lens Stiffness Changes

Published on: April 5, 2024

Related Experiment Videos

Last Updated: Apr 30, 2026

Author Spotlight: Enhancing Visual Outcomes in Cataract Surgery: A Novel Technique to Prevent Posterior Capsular Opacification Through IOL Rotation

Author Spotlight: Enhancing Visual Outcomes in Cataract Surgery: A Novel Technique to Prevent Posterior Capsular Opacification Through IOL Rotation

Published on: July 7, 2023

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery

Published on: December 1, 2023

Author Spotlight: Advancing Understanding of Age-Related Lens Stiffness Changes

Author Spotlight: Advancing Understanding of Age-Related Lens Stiffness Changes

Published on: April 5, 2024

Main Results:

The LMP-agarose gel provided stable support for the capsular bag, enabling clear microscopic observation and whole-mount immunostaining.
Proliferation and migration of LECs onto the posterior capsule were observed, with epithelial cells appearing at the central portion after approximately 56.6 hours (rodent) and 106 hours (porcine).
Histological analysis confirmed LEC proliferation, migration, and transdifferentiation, consistent with PCO development.

Conclusions:

The developed LMP-agarose gel-based in vitro model effectively supports the capsular bag and allows for detailed investigation of PCO.
This model maintains the natural contour of the capsule without internal or external implants, facilitating pharmacological and histological evaluations.
This system offers a valuable tool for PCO research, drug screening, and understanding the cellular mechanisms underlying this post-surgical complication.