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Related Concept Videos

DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
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High-quality DNA from fingernails for genetic analysis.

Sandra Preuner1, Martin Danzer2, Johannes Pröll2

  • 1Children's Cancer Research Institute, Vienna, Austria; Labdia Labordiagnostik GmbH, Vienna, Austria.

The Journal of Molecular Diagnostics : JMD
|May 6, 2014
PubMed
Summary

Fingernail clippings can yield high-quality DNA for advanced genetic testing. A refined protocol using the Prepfiler Forensic DNA Extraction kit optimizes DNA yield and integrity for applications like next-generation sequencing.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Forensic Science

Background:

  • High-quality germline DNA is crucial for genetic analyses.
  • Fingernail clippings are a viable source of constitutional DNA for PCR.
  • Existing nail DNA extraction protocols often yield insufficient DNA quality and quantity for advanced applications like next-generation sequencing (NGS).

Purpose of the Study:

  • To systematically optimize DNA purification protocols from fingernail clippings.
  • To enhance DNA yield and quality for demanding downstream genetic analyses.
  • To establish a reliable method for extracting DNA from nail material for research and diagnostics.

Main Methods:

  • Extensive testing and modification of various DNA purification protocols from nail material.
  • Assessment of DNA integrity using PCR amplification of short, mid-range, and long-range DNA fragments.
  • Evaluation of the Prepfiler Forensic DNA Extraction kit for DNA isolation from fingernail clippings.
  • Demonstration of suitability for next-generation sequencing (NGS) via high-resolution HLA-typing.

Main Results:

  • The Prepfiler Forensic DNA Extraction kit provided the highest yield and quality of DNA from nail clippings.
  • A standardized input of 20 mg nail material yielded a mean of 1 μg of DNA.
  • High amplifiability was observed for short (93%) and mid-range (90%) DNA targets, and long-range targets (60%).
  • Successful high-resolution HLA-typing was achieved, demonstrating the protocol's adequacy for NGS applications.

Conclusions:

  • The optimized protocol effectively isolates high-quality DNA from fingernail clippings.
  • This method enables the use of fingernail material for demanding genomic analyses, including NGS.
  • The protocol supports advancements in both genetic research and diagnostic applications.