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Related Concept Videos

SDS-PAGE01:27

SDS-PAGE

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Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
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Capillary Electrophoresis: Applications01:30

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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Analyte Adsorption and Distribution01:09

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In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

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Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
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A dextran-bonded stationary phase for saccharide separation.

Qianying Sheng1, Xindai Su2, Xiuling Li3

  • 1Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.

Journal of Chromatography. A
|May 6, 2014
PubMed
Summary

A novel saccharide-based stationary phase for hydrophilic interaction liquid chromatography (HILIC) was developed using a simple dextran bonding method. This new HILIC stationary phase offers excellent separation efficiency and stability for polar compounds and carbohydrates.

Keywords:
DextranGlycopeptidesHILICMSOligosaccharide

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Materials Science

Background:

  • Hydrophilic interaction liquid chromatography (HILIC) is crucial for separating polar compounds.
  • Traditional HILIC stationary phases often involve complex immobilization procedures.
  • Developing efficient and stable HILIC stationary phases remains an active research area.

Purpose of the Study:

  • To present a novel saccharide-based stationary phase for HILIC.
  • To develop a simple and efficient method for immobilizing dextran onto silica gel.
  • To evaluate the performance of the new stationary phase for separating polar compounds and carbohydrates.

Main Methods:

  • Utilizing carbonyl di-imidazole (CDI) as a cross-linker for dextran in aqueous solution.
  • Bonding high molecular weight polysaccharide (dextran) onto silica gel.
  • Evaluating separation efficiency, column stability, and retention mechanisms for various analytes.

Main Results:

  • A simple and effective method for creating a dextran-bonded HILIC stationary phase was achieved.
  • The new phase demonstrated good separation efficiency and column stability for polar compounds and carbohydrates.
  • The retention mechanism aligned with typical HILIC behavior, and good selectivity was observed for oligosaccharides and glycopeptides.

Conclusions:

  • The developed dextran-bonded stationary phase offers a promising alternative for HILIC applications.
  • This method provides a straightforward approach to creating robust and efficient HILIC columns.
  • The phase shows potential for the analysis of complex mixtures containing carbohydrates and glycopeptides.