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Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

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RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
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Translesion DNA Polymerases02:10

Translesion DNA Polymerases

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Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
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Lysogenic Cycle of Bacteriophages00:43

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In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
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Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
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Updated: Feb 6, 2026

Quantitative PCR of T7 Bacteriophage from Biopanning
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Quantitative PCR of T7 Bacteriophage from Biopanning

Published on: September 27, 2018

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Bacteriophage T7 DNA polymerase - sequenase.

Bin Zhu1

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School Boston, MA, USA.

Frontiers in Microbiology
|May 6, 2014
PubMed
Summary
This summary is machine-generated.

The T7 bacteriophage DNA polymerase, engineered into Sequenase, revolutionized DNA sequencing by efficiently incorporating modified nucleotides. Its high fidelity and processivity made it ideal for developing sequencing technologies.

Keywords:
DNA polymeraseDNA sequencingbacteriophage T7marine phagessequenase

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Quantitative PCR of T7 Bacteriophage from Biopanning
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Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Enzymology

Background:

  • DNA sequencing technology relies on enzymes with specific characteristics for efficient and accurate nucleotide incorporation.
  • Bacteriophage T7 DNA polymerase possesses inherent properties suitable for chain-terminating DNA sequencing.

Purpose of the Study:

  • To review the historical development of T7 DNA polymerase for DNA sequencing applications.
  • To highlight key advancements in modifying T7 DNA polymerase for biotechnological use.

Main Methods:

  • Review of historical studies on T7 DNA polymerase.
  • Analysis of enzyme characteristics relevant to DNA sequencing.
  • Examination of modifications leading to Sequenase.

Main Results:

  • T7 DNA polymerase exhibits ideal features for DNA sequencing: efficient modified nucleotide incorporation, high processivity, and high fidelity.
  • Engineering of T7 DNA polymerase resulted in Sequenase, significantly advancing DNA sequencing technology.
  • Lessons learned from T7 DNA polymerase inform the study of novel DNA polymerases.

Conclusions:

  • T7 DNA polymerase and its engineered form, Sequenase, were pivotal in advancing DNA sequencing.
  • The study of T7 DNA polymerase provides a model for discovering and modifying enzymes for biotechnology.