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Proteins: From Genes to Degradation02:11

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Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
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Updated: Apr 30, 2026

Assays for the Degradation of Misfolded Proteins in Cells
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Assays for the Degradation of Misfolded Proteins in Cells

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When RNA and protein degradation pathways meet.

Benoît Derrien1, Pascal Genschik2

  • 1Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357, Conventionné avec l'Université de Strasbourg Strasbourg, France.

Frontiers in Plant Science
|May 6, 2014
PubMed
Summary

RNA silencing, crucial in eukaryotes, is regulated by protein degradation pathways. This review focuses on proteasomal and autophagic control of key RNA silencing factors, particularly in plants.

Keywords:
RISCRNA silencingautophagyproteasomeubiquitinvirus

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Area of Science:

  • Molecular Biology
  • Biomedical Research
  • Genetics

Background:

  • RNA silencing is a conserved eukaryotic mechanism regulating development, transposition, heterochromatin, and antiviral defense.
  • The regulatory mechanisms governing the RNA silencing machinery itself are not well understood.
  • Recent studies indicate that key RNA silencing components are subject to proteasomal and autophagic degradation.

Purpose of the Study:

  • To review post-translational proteolytic regulations of RNA silencing machinery.
  • To emphasize the role of these regulations in plant research.
  • To discuss the functional relevance of these regulatory pathways.

Main Methods:

  • Literature review of recent reports on RNA silencing regulation.
  • Focus on proteasomal and autophagic pathways.
  • Emphasis on studies conducted in plant systems.

Main Results:

  • Key RNA silencing components, including ARGONAUTE proteins and Dicer, are regulated by proteasomal and autophagic degradation.
  • These proteolytic pathways play a significant role in controlling the abundance and activity of RNA silencing factors.
  • Proteolytic regulation is essential for the proper functioning of RNA silencing in various biological processes.

Conclusions:

  • Post-translational proteolytic regulation is a critical aspect of RNA silencing machinery control.
  • Understanding these pathways, especially in plants, is vital for comprehending gene regulation and defense mechanisms.
  • Further research into these regulatory networks will uncover new therapeutic targets and insights into fundamental biological processes.