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Molecular cloning and characterization of cDNA encoding mouse cytokeratin no. 19

Y Ichinose1, K Hashido, H Miyamoto

  • 1Department of Microbial Genetics, Osaka University, Japan.

Gene
|August 15, 1989
PubMed

Insights

Researchers isolated mouse cytokeratin 19 (Ck19) cDNA, revealing a protein structure similar to human and bovine Ck19. Mouse Ck19 mRNA expression was observed in adult tissues and specific cell lines, suggesting potential roles in development.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Developmental Biology

Background:

  • Cytokeratins (Ck) are intermediate filament proteins crucial for cellular structure.
  • Cytokeratin 19 (Ck19) is a type I keratin found in various epithelial tissues.
  • Understanding the structure and expression of Ck19 is important for cell biology and disease research.

Purpose of the Study:

  • To isolate and characterize cDNA clones encoding mouse cytokeratin 19 (Ck19).
  • To analyze the deduced amino acid sequence and compare it with homologous proteins.
  • To investigate the expression pattern of mouse Ck19 mRNA in different tissues and cell lines.

Main Methods:

  • Isolation of cDNA clones from a mouse intestinal cDNA library using synthetic oligodeoxyribonucleotides.
  • Nucleotide sequence analysis to determine protein size, amino acid composition, and structural domains.
  • Northern blot analysis to detect and quantify Ck19 mRNA expression.

Main Results:

  • Four independent cDNA clones encoding mouse Ck19 were isolated, corresponding to a 1.4-kb cDNA.
  • The deduced protein (44,541 Da, 403 amino acids) shares high overall amino acid identity (82.7-82.4%) with human and bovine Ck19.
  • Mouse Ck19 mRNA is highly expressed in adult intestine, stomach, and uterus, and in a placental cell line and F9 teratocarcinoma cells, but not in PYS-2 cells.

Conclusions:

  • Mouse Ck19 exhibits structural similarities to other mammalian Ck19 proteins, particularly in its central alpha-helical domain.
  • The expression pattern of mouse Ck19 suggests a role in specific epithelial tissues and developmental processes.
  • The observed expression profile in F9 cells and its similarity to EndoC expression indicate a potential shared regulatory mechanism or function.

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