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1F7, a novel cell surface molecule, involved in helper function of CD4 cells
C Morimoto1, Y Torimoto, G Levinson
1Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.
Journal of Immunology (Baltimore, Md. : 1950)
|December 1, 1989
Summary
A new antibody, anti-1F7, targets a specific antigen (1F7 Ag) on T cells, impacting T cell proliferation and B cell antibody synthesis. This discovery helps differentiate T cell subsets involved in immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- T cell proliferation and B cell antibody synthesis are crucial immune responses.
- Specific cell surface antigens play key roles in regulating these processes.
Purpose of the Study:
- To characterize a novel monoclonal antibody, anti-1F7, and its target antigen (1F7 Ag).
- To investigate the role of 1F7 Ag in T cell subsets and immune functions.
Main Methods:
- Development of anti-1F7 monoclonal antibody.
- Flow cytometry analysis of T cell populations (CD4+, CD8+, CDw29+).
- Assessment of T cell proliferation and B cell IgG synthesis.
Main Results:
- Anti-1F7 inhibits T cell proliferation and IgG synthesis.
- 1F7 Ag is expressed on T cells, particularly on CD4+CDw29+ helper T cells.
- Anti-1F7 further subdivides CD4+CDw29+ cells into 1F7+ and 1F7- subsets.
- The CD4+CDw29+1F7+ population shows maximal proliferation to recall antigens.
- Helper function for IgG synthesis involves both CD4+CDw29+1F7+ and CD4+CDw29+1F7- cells.
- Anti-1F7 defines a 110-kDa molecule related to the T cell activation antigen Ta1.
Conclusions:
- The anti-1F7 antibody identifies a novel antigen (1F7 Ag) on T cells.
- 1F7 Ag expression helps delineate functional T cell subsets involved in adaptive immunity.
- This antibody provides a new tool for studying T cell regulation and immune responses.