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Antipeptide antibodies that recognize a lymphocyte substance P receptor
D W Pascual1, J E Blalock, K L Bost
1Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
Journal of Immunology (Baltimore, Md. : 1950)
|December 1, 1989
Summary
Researchers developed novel antibodies to identify substance P (SP) receptors on lymphocytes. These antibodies successfully recognized and isolated a specific SP receptor protein from IM-9 cells, confirming its presence on the lymphocyte surface.
Area of Science:
- Immunology
- Neuroscience
- Cell Biology
Background:
- Substance P (SP) is a neuropeptide involved in various physiological processes.
- The presence and function of SP receptors on lymphocytes are not fully understood.
- Investigating SP receptors on lymphocytes could reveal new therapeutic targets.
Purpose of the Study:
- To develop and validate antibodies for detecting SP receptors on lymphocytes.
- To characterize the SP receptor expressed on the IM-9 lymphoblast cell line.
Main Methods:
- Generation of polyclonal antibodies against a substance P complementary peptide (SP CP).
- Utilizing IM-9 lymphoblast cell line for antibody validation.
- Immunoaffinity chromatography and Western blot analysis for protein isolation and identification.
Main Results:
- Antibodies against SP CP specifically recognized a molecule on the surface of IM-9 cells.
- This molecule's binding of SP was competitively inhibited by SP antagonists.
- A 58,000 D protein, identified as an SP receptor, was isolated and characterized.
Conclusions:
- Antibodies against SP CP effectively identify a substance P receptor on the IM-9 lymphocyte cell line.
- This study provides evidence for the presence of functional SP receptors on lymphocytes.
- The developed antibodies are valuable tools for further research into SP receptor function in immune cells.