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Updated: Apr 30, 2026
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Comparison of three molecular assays for the detection of rifampin resistance in Mycobacterium tuberculosis
Hee-Won Moon1, Mina Hur, Ji-Young Kim
1Department of Laboratory Medicine, Konkuk University School of Medicine, Konkuk University Hospital, Seoul, Korea.
Background:
Rifampin (RIF) is the most important first-line antituberculosis drug, and resistance to this drug may result in treatment failures. We evaluated the diagnostic performances of recently introduced, molecular assays for the detection of RIF resistance.
Methods:
A total of 100 isolates (50 RIF resistant and 50 RIF susceptible) were studied. Their RIF resistances were determined by conventional drug-susceptibility test. These results were compared with those of three molecular assays: Xpert MTB/RIF assay (MTB is Mycobacterium tuberculosis), Sacace MTB Real-TM resistance, and AdvanSure MDR-TB GenoBlot assay (MDR is multidrug resisitant).
Results:
Sensitivities for RIF resistance detection of Xpert MTB/RIF assay, Sacace MTB Real-TM resistance, and Advansure GenoBlot assay were 94.0%, 91.8%, and 84.0%, respectively. Their specificities for RIF resistance detection were all 100%.
Conclusion:
Three molecular assays for the detection of RIF resistance have various performances. Xpert MTB/RIF assay shows the highest sensitivity among the three molecular assays and can be an effective choice in clinical laboratories.
