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Short communication: Viable Mycobacterium avium subspecies paratuberculosis in retail artisanal Coalho cheese from

A C S Faria1, D G G Schwarz2, I A Carvalho2

  • 1Faculdade de Ciências Biológicas e da Saúde (FACISA/UNIVIÇOSA), Av. Maria de Paula Santana, 3815, Silvestre, CEP 36570-000, Viçosa, Minas Gerais, Brazil.

Journal of Dairy Science
|May 7, 2014
PubMed

Insights

The first detection of Mycobacterium avium ssp. paratuberculosis (MAP) in Brazilian Coalho cheese confirms its presence in retail products. Viable MAP cells were recovered, highlighting potential public health concerns from contaminated dairy. Keywords: MAP, paratuberculosis, Coalho cheese, dairy products, Brazil.

Area of Science:

  • Food Microbiology
  • Veterinary Medicine
  • Public Health

Background:

  • Mycobacterium avium ssp. paratuberculosis (MAP) causes paratuberculosis and is implicated in Crohn's disease.
  • Contaminated milk and dairy products are potential human transmission routes for MAP.
  • MAP has been found in cheeses globally, necessitating investigation in regional products like Brazilian Coalho cheese.

Purpose of the Study:

  • To report the initial detection of MAP in retail Coalho cheese within Brazil.
  • To identify viable MAP cells and confirm their genetic identity in the analyzed cheese samples.

Main Methods:

  • Analysis of 30 retail Coalho cheese samples.
  • Polymerase Chain Reaction (PCR) for MAP DNA amplification.
  • Bacterial culture for isolating viable MAP.
  • DNA sequencing of positive cultures for genetic identification (IS900 insertion sequence).

Main Results:

  • MAP-specific DNA fragments were detected in 10% (3/30) of samples via PCR.
  • Viable MAP was successfully cultured from 3.3% (1/30) of the retail Coalho cheese samples.
  • Sequencing confirmed 99% identity of the isolated MAP DNA with the IS900 sequence in GenBank.

Conclusions:

  • This study provides the first evidence of MAP DNA and viable MAP presence in retail Coalho cheese in Brazil.
  • The findings indicate a potential risk associated with consuming this popular Brazilian dairy product.
  • Further research is warranted to assess the prevalence and public health implications of MAP in Brazilian dairy products.
Keywords:
cheesecultureparatuberculosisviable

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